LymphedemaV1, Main, Exploration, bibRecord, 004362

Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function

Identifieur interne : 004362 ( Main/Exploration ); précédent : 004361; suivant : 004363

Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function

Auteurs : Timothy Kassis [Géorgie (pays)] ; Alison B. Kohan [États-Unis] ; Michael J. Weiler [Géorgie (pays)] ; Matthew E. Nipper [Géorgie (pays)] ; Rachel Cornelius [Géorgie (pays)] ; Patrick Tso [États-Unis] ; J. Brandon Dixon [Géorgie (pays)]

Source :

Journal of Biomedical Optics [ 1083-3668 ] ; 2012.

RBID : PMC:3413897

Abstract

Abstract.

Nearly all dietary lipids are transported from the intestine to venous circulation through the lymphatic system, yet the mechanisms that regulate this process remain unclear. Elucidating the mechanisms involved in the functional response of lymphatics to changes in lipid load would provide valuable insight into recent implications of lymphatic dysfunction in lipid related diseases. Therefore, we sought to develop an in situ imaging system to quantify and correlate lymphatic function as it relates to lipid transport. The imaging platform provides the capability of dual-channel imaging of both high-speed bright-field video and fluorescence simultaneously. Utilizing post-acquisition image processing algorithms, we can quantify correlations between vessel pump function, lymph flow, and lipid concentration of mesenteric lymphatic vessels in situ. All image analysis is automated with customized LabVIEW virtual instruments; local flow is measured through lymphocyte velocity tracking, vessel contraction through measurements of the vessel wall displacement, and lipid uptake through fluorescence intensity tracking of an orally administered fluorescently labelled fatty acid analogue, BODIPY FL C16. This system will prove to be an invaluable tool for scientists studying intestinal lymphatic function in health and disease, and those investigating strategies for targeting the lymphatics with orally delivered drugs to avoid first pass metabolism.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413897

DOI: 10.1117/1.JBO.17.8.086005
PubMed: 23224192
PubMed Central: 3413897

Affiliations:

Le document en format XML

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 optical imaging system for quantifying lipid uptake and lymphatic pump function</title>
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 optical imaging system for quantifying lipid uptake and lymphatic pump function</title>
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<p>Nearly all dietary lipids are transported from the intestine to venous circulation through the lymphatic system, yet the mechanisms that regulate this process remain unclear. Elucidating the mechanisms involved in the functional response of lymphatics to changes in lipid load would provide valuable insight into recent implications of lymphatic dysfunction in lipid related diseases. Therefore, we sought to develop an <italic>in situ</italic>
 imaging system to quantify and correlate lymphatic function as it relates to lipid transport. The imaging platform provides the capability of dual-channel imaging of both high-speed bright-field video and fluorescence simultaneously. Utilizing post-acquisition image processing algorithms, we can quantify correlations between vessel pump function, lymph flow, and lipid concentration of mesenteric lymphatic vessels <italic>in situ</italic>
. All image analysis is automated with customized LabVIEW virtual instruments; local flow is measured through lymphocyte velocity tracking, vessel contraction through measurements of the vessel wall displacement, and lipid uptake through fluorescence intensity tracking of an orally administered fluorescently labelled fatty acid analogue, BODIPY FL <inline-formula><mml:math id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">C</mml:mi>
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. This system will prove to be an invaluable tool for scientists studying intestinal lymphatic function in health and disease, and those investigating strategies for targeting the lymphatics with orally delivered drugs to avoid first pass metabolism.</p>
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Serveur d'exploration sur le lymphœdème

Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function

Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function

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Abstract

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